ABSTRACT
CAT3 is a promising anti-brain tumor agent that has significant anti-tumor activity on Daoy or U87MG orthotopic xenograft in nude mice. This study was carried out to investigate the metabolic profiles of CAT3 in mouse/dog/human blood and microsome as well as in humanized recombinant enzymes. All animal care and experimental procedures were reviewed and approved by the Animal Ethics Committee of Chinese Academy of Medical Sciences. Our findings showed that CAT3 could be hydrolyzed to active metabolite PF403 by carboxylesterase, butyrylcholinesterase and serine hydrolase in mouse/dog/ human blood. PF403 could be further metabolized to M1 oxidative dehydration product, M2 double oxidation dehydration product, M3 methylation oxidative dehydration product, M4 oxidation product and M5 demethylation product, which were mainly catalyzed by CYP1A2, 1A1, 2C9 and 3A4, and slightly by CYP2B6, 2C8, 2C19 and 2D6. Besides oxidative metabolism, PF403 also was transformed into glucuronylation metabolites GLU-PF403 by Phase II enzymes UGT1A1, 1A3 and 1A9. Taken together, the metabolism of CAT3 was a multiple enzyme catalytic reaction. These results could provide valuable information for potential enzyme-mediated DDI in clinic studies.
ABSTRACT
" 's triple nine needling therapy" is the crucial acupuncture prescription in treatment of eye diseases in 's academic school of ophthalmology. " 's triple nine needling therapy" includes the three points near to the eyes, the three groups of points for penetrating acupuncture around the eyes and the acupoint selection based on the general differentiation of syndrome. In this paper, the acupoint selection and the thinking of acupoint combination were introduced in the treatment of optic nerve disease on the base of the theory of " 's triple nine needling" prescription. The specific needling manipulations at different regions involved in the triple needling procedure were explained in detail. It is proposed that the acupoints are combined and the correct needling manipulations selected rationally in compliance with the illness condition and the syndrome characteristics to ensure maximally the clinical effects of " 's triple nine needling therapy".
Subject(s)
Humans , Acupuncture Therapy , Needles , Optic Nerve Diseases , TherapeuticsABSTRACT
Objective To observe the clinical outcomes of corneal collagen cross-linlIng (CLX) and laser-assisted in situ keratomileusis (LASIK) for refractive error.Methods Together 100 patients (200 eyes) with myopia and/or astigmatism who underwent LASIK were admitted from December 2015 to January 2016,and divided into treatment group (50 patients,100 eyes),in which patient received CXL-LASIK,and control group (50 patients,100 eyes),in which the controls were given LASIK alone.Then the optical variables,including visual acuity,diopter,cornea endothelium cell counting,corneal transparency,corneal collagen cross-linking line,were examined and analyzed before operation and 1 week,1 month,3 months,6 months and 12 months after opera tion.Results As for postoperative time points,the uncorrected visual acuity after operation was better than the best corrected visual acuity before surgery in the two groups,but the differences in diopter between the two groups were statistically significant (all P ＜ 0.05).There was no significant difference in diopter 1 month and 12 months postoperatively in the treatment group (t =0.021,P =0.070),while its difference in the control group 1 month and 12 months postoperatively(t =4.857,P =0.010).The differences of the cornea endothelium cell counting in the treatment group between before and after surgery were not statistically significant (F =1.163,P =0.327).Moreover,slightly opacification apPeared in anterior corneal stromal in the early stage after surgery in the treatment group,and the average thickness of corneal collagen cross-linking line was 210.00-340.00 (270.48 + 37.80) μm 1 month postoperatively,accounting for 63％ of the central cornea thickness.There were not flap-related complications in the treatment group,but the control group had corneal flap wrinkle in 3 eyes and displacement in 1 eye in the controls.Conclusion Corneal collagen cross-linlIng and laser-assisted in situ keratomileusis for refractive error can obtain better outcomes,including sound uncorrected visual acuity and stable diopter after surgery,without the loss of the corneal endothelial cells.
ABSTRACT
Objective To observe the clinical outcomes of corneal collagen cross-linlIng (CLX) and laser-assisted in situ keratomileusis (LASIK) for refractive error.Methods Together 100 patients (200 eyes) with myopia and/or astigmatism who underwent LASIK were admitted from December 2015 to January 2016,and divided into treatment group (50 patients,100 eyes),in which patient received CXL-LASIK,and control group (50 patients,100 eyes),in which the controls were given LASIK alone.Then the optical variables,including visual acuity,diopter,cornea endothelium cell counting,corneal transparency,corneal collagen cross-linking line,were examined and analyzed before operation and 1 week,1 month,3 months,6 months and 12 months after opera tion.Results As for postoperative time points,the uncorrected visual acuity after operation was better than the best corrected visual acuity before surgery in the two groups,but the differences in diopter between the two groups were statistically significant (all P ＜ 0.05).There was no significant difference in diopter 1 month and 12 months postoperatively in the treatment group (t =0.021,P =0.070),while its difference in the control group 1 month and 12 months postoperatively(t =4.857,P =0.010).The differences of the cornea endothelium cell counting in the treatment group between before and after surgery were not statistically significant (F =1.163,P =0.327).Moreover,slightly opacification apPeared in anterior corneal stromal in the early stage after surgery in the treatment group,and the average thickness of corneal collagen cross-linking line was 210.00-340.00 (270.48 + 37.80) μm 1 month postoperatively,accounting for 63％ of the central cornea thickness.There were not flap-related complications in the treatment group,but the control group had corneal flap wrinkle in 3 eyes and displacement in 1 eye in the controls.Conclusion Corneal collagen cross-linlIng and laser-assisted in situ keratomileusis for refractive error can obtain better outcomes,including sound uncorrected visual acuity and stable diopter after surgery,without the loss of the corneal endothelial cells.
ABSTRACT
During the process of electroacupuncture (EA) therapy, whether there being a corrosive effect in ac- upuncture needles was observed. Acupuncture needles were inserted into a rabbit's acupoint to perform a 12-hour electrical stimulation with three types of common EA waveform; additionally two needles were put in 0.9% sodium chloride solution with 12-hour direct current. Afterwards, environmental scanning electron microscope was applied to detect the surface physical characteristics of acupuncture needles. As a result, after a 12-hour continued electri- cal stimulation with three types of common EA waveform in the rabbit, there was no corrosive effect in acupunc- ture needles; but the direct current could cause severe corrosion in acupuncture needles. It is believed that there is no corrosion effect on acupuncture needles in current EA treatment, and some accidents reported in literature may be related to quality of EA device or improper manipulation during the treatment.
Subject(s)
Animals , Male , Rabbits , Acupuncture Points , Corrosion , Electroacupuncture , NeedlesABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of sevoflurane on membrane permeability of alveolar capillaries in rats with acute lung injury and the ratio of inflammatory cells in bronchoalveolar lavage fluid in rats with acute endotoxin lung injury.</p><p><b>METHODS</b>48 Wistar rats were selected and divided into group C, L, S1L and S2L after injection evans blue 50 mg/kg in random with 12 rats in each group. Group C was taken as control group, 1.2 ml normal saline was injected into the rats via femoral vein and then the rats were mechanically ventilated for 4 hours; The rats in group L were also mechanically ventilated for 4 hours after injection of endotoxin 5 mg/kg via the same vein. For the rats in group S1L and S2L, 1.0 or 1.5 minimal alveolar concentration (MAC) sevoflurane was inhaled with mechanical ventilation after injection of endotoxin 5 mg/kg. Evans blue was not injected into 6 rats of each group in order that the 6 rats could be used for pathological examination and alveoli lavage, lung pathomorphological score of the lung, lung wet/dry weight ratio, the content of lung water, lung permeability index, content of evans blue, total amount and ratio of inflammatory cells in bronchoalveolar lavage fluid (BALF) were all determined.</p><p><b>RESULTS</b>Sevoflurane of 1.0 MAC and 1.5 MAC made lung wet/dry weight ratio and content of lung water change insignificantly; lung permeability index, content of evans blue and pathomorphological score in group S1L decreased from 4.86 +/- 0.82, 112.21 +/- 11.44 ng/mg, 9.17 +/- 0.90 to 3.98 +/- 0.50, 92.85 +/- 11.80 ng/mg, 7.50 +/- 0.96; group S2L decreased to 3.91 +/- 0.34, 96.33 +/- 8.79 ng/mg, 7.67 +/- 0.75. Sevoflurane of 1.0 MAC and 1.5 MAC did not have a significantly effect on total amount and ratio of inflammatory cells in BALF.</p><p><b>CONCLUSION</b>Membrane permeability of alveolar capillaries after acute endotoxin lung injury decreased by inhalation of sevoflurane of 1.0 MAC and 1.5 MAC and pathological injury of lung tissue relieved.</p>